利用丛生芽增殖途径对大花蕙兰进行离体快繁

In vitro Propagation of Cymbidium via Clustered Shoots

以大花蕙兰的侧芽为外植体,研究了利用丛生芽途径快速繁殖大花蕙兰的技术。结果表明:通过丛生芽增殖途径也能达到快速繁殖大花蕙兰的目的,在1/2MS+6-BA3.5mg·L-1+NAA0.2mg·L-1+卡拉胶8g·L-1+蔗糖30g·L-1+φ=10%的椰子水(CW)或w=10%的香蕉匀浆的培养基上,丛生芽增殖较快,以40d为1个继代周期,1年最少可增殖9次,以每个培养周期增殖3.6倍计,1个芽1年可繁殖出3.69≈10万株苗;在丛生芽增殖培养过程中,会出现褐化现象,但并未对大花蕙兰丛生芽的增殖产生不良影响;添加φ=10%的CW或w=10%的香蕉匀浆有利于提高丛生芽的增值率及其生长势;试管苗在1/2MS+NAA1.0mg·L-1+AC1g·L-1+卡拉胶8g·L-1+蔗糖30g·L-1+φ=10%的CW的培养基上生根良好,生根率为86.1%;试管苗移栽在水苔上的成活率达92.5%。

Lateral buds of Cymbidium were used as explants to induce to form clustered shoots or multiple shoot clumps for rapid propagation of Cymbidium. The multiple shoot clumps were proliferated much faster when cul- tured on 1/2MS＋ 6-BA3.5 mg·L-1 ＋NAA0.2 mg·L-1 ＋10% coconut water （CW） （or 10% banana homogenate） medium, and proliferation could be done at least 9 times a year when a subculture cycle took 40 days to complete, or a lateral bud can produce 3.69（ about 100 thousand） plants a year. Browning occurred oc- casionally in multiple shoot clumps during the subculture but did not give negative impact on the proliferation of the multiple shoot clumps. Addition of 10% coconut water （CW） （or 10% banana homogenate） into the bud proliferation medium gave a higher proliferation rate of multiple shoot clumps and improved their growth vigor. The test tube plantlets were rooted well with the rooting rate of 86.1% when cultured on the rooting medium of 1/2MS ＋NAA1.0 mg ·L-1 ＋1.0 g·L-1 AC ＋10% CW. The rooted plantlets had a survival rate of upto 92. 5 % when transferred and cultured on the sphagnum.