吉西他滨致轻度胰腺癌相关恶病质模型的建立

Establishment of Gemcitabine-induced mild cachexia model with pancreatic cancer

目的探讨吉西他滨致胰腺癌轻度恶病质模型的制备方法。方法 24只裸鼠胰腺癌皮下移植瘤模型随机平均分为G0组、G3组(吉西他滨50mg/kg,腹腔内给药,术后第1、4、7天)、G4组(吉西他滨50mg/kg,腹腔内给药,术后第1、4、7、10天),实验共25d。比较3组动物体质量、进食量、肿瘤体积、皮下脂肪及臂围的变化及其时-效关系。结果 G3组和G4组与G0组在体质量、进食量、肿瘤体积、皮下脂肪及臂围方面,比较差异均有统计学意义(P<0.05)。第13天时,G3组与G4组动物的平均体质量下降分别为9.7%、14.1%;第14天时,G4组的日均进食量[(3.38±0.14)g]显著低于G3组[(3.59±0.12)g,P<0.05]。结论不同剂量的吉西他滨对裸鼠胰腺癌模型的进食和体质量具有显著影响,其中吉西他滨(50mg/kg,腹腔内给药,术后第1、4、7天)为制备轻度胰腺癌相关恶病质模型的适宜剂量。

Objective To investigate the establishment of mild cachexia model by using Gemcitabine in pancreatic tumor-bearing mice. Methods 24 xenograft nude mice were randomly divided into GO group,G3 group（given 50 mg/kg of Gemcitabine intraper itoneal on days 1,4,7 after transplantation） and G4 group（given 50 mg/kg of Gemcitabine intraperitoneal on days 1,4,7,10 after transplantation） by average. Body weight, food intake, tumor size, subcutaneous fat,arm circumference and the time-effect relation- ship were compared among the three groups. Results There were significant differences in body weight, food intake, tumor size, subcutaneous fat and arm circumference between chemotherapy groups and control group（P〈0.05）. At 13 d, the weight loss of G3 and G4 groups were 9.7 % and 14.1% respectively. At 14 d, the food intake of G3 gruop[ （3.59 ± 0.12） g] was significantly higher than that of G4 gruop[（3.38±0.14）g,P〈0.05]. Conclusion Different doses of Gemcitabine had a significant impact on the body weight and food intake of the xenograft nude mice with pancreatic cancer. The optimum dose of Gemcitabine to establish chemother apy-induced mild cachexia model in pancreatic cancer mouse was intraperitoneal injection Gemcitabine（50 mg/kg） on days 1,4,7.