早花及晚花灰毡毛忍冬中绿原酸含量分析

Analysis on the Content of Chlorogenic Acid from Early-flowering and Later-flowering Lonicera Macranthoides

研究早花和晚花灰毡毛忍冬花蕾及叶中绿原酸含量，筛选适宜贵州北部推广种植优良品种，采用超声波辅助提取灰毡毛忍冬中的绿原酸，高效液相色谱法测定其含量，色谱条件为：Diamonsil柱（5μm，C18（2），250mm×4．6mm），甲醇／乙腈（5：11）-0．1％磷酸=20：80，流速1．0mL／min，柱温40℃，检测波长325nm。结果表明旱花品种花蕾中绿原酸含量为2．36％～3．44％，叶中绿原酸含量为0．49％o～1．11％；晚花花蕾中绿原酸含量为2．60％～4．07％，叶中绿原酸含量为1．92％．2．83％。因此晚花衣毡毛忍冬是一种富绿原酸育种系。

To explore suitable planting fine Lonicera macranthoides varieties in north Guizhou, the content of chlorogcnic acid in flower buds and leaves of early-flowering and later-flowering varieties were researched. Chlorogenic acid were obtained with ultrasonnic wave-assisted extraction technique, and analyzed using HPLC methods with operating condition as foUows： Diamonsil （5 μm, C18（2）, 250 mm×4.6 mm）,the volume flow 1.0mL/min, the column temperature 40℃ and the detection wavelength 325nm, The mobile phase methanol/acetonitrile （5：11）-0.1% phosphoric acid=20：80. The result showed that the content of chlorogenic acid ranged from 2.36% to 3.44% in flower buds and from 0.49% to 1.11% in leaves of early-flowering Lonicera macranthoides. In flower buds and leaves of later-flowering one, it as in the range of 2.60%,～4.07% and 1.92%～2.83 %, respectively. It was concluded that the later-flowering Lonicera macranthoide was a rich-ehlomgenic acid breeding sWairL