芽胞杆菌资源库管理系统的构建

Construction of Resource Database Management Cystem of Bacillus

建立芽胞杆菌菌种资源库的收集保藏系统和以脂肪酸、rRNA和传统生理生化特性的细菌鉴定系统,以气质联用、液质联用鉴定结果构建细菌次生代谢物库;在此基础上建立一整套针对作物病害、虫害、线虫为害以及杂草的微生物农药候选菌株的筛选评价系统。通过建立这套系统,能够大大加快菌株的筛选,尽快将有用的菌株从保存转入前期研究阶段,对存在重大潜在应用价值的菌株的生理生化、发酵培养等特性进行基础性研究,使其能够尽快地进入开发阶段,为相关菌株的产业化做好充分的准备。

Bacillus has very good biological characteristics due to its stability and high resistance,and then has been widely applied in industry,agriculture and medicine.Collection,preservation,appraisal system and the database of secondary metabolites of Bacillus strains were constructed.The Appraisal System contains the information of fatty acid,rRNA and physiological and biochemical property of Bacillus stains.The data of Secondary Metabolites were obtained from the GC-MS and LC-MS measurements.Then,a screening and evaluation system of microbial pesticides candidate strains will be formed,which can accelerate the screening of Bacillus strains.And then,researching on the strains which may have good potential application will be carried out rapidly and get ready for the industrialization.