发酵蔗渣产黄腐酸菌种的筛选及应用

Screening and Application of Fermentation Strains for Decomposing Bagasse to Produce Fulvic Acid

为提高蔗渣发酵产黄腐酸的能力,从生物腐植酸样品中分离培养菌种,对分离获得的菌种进行较高温下降解纤维素能力的测定,初步筛选出生长良好和降解纤维素能力较强的菌株,鉴别其形态、并接种于蔗渣物料发酵试验进一步筛选,最后将优化筛选的菌株进行16sDNA鉴定,同时应用于工厂生产性装袋的中试发酵。结果表明腐植酸分离样品中芽孢杆菌占绝对优势,其中芽孢杆菌E菌株具有较高的转化蔗渣产黄腐酸的能力。该菌株经16sDNA鉴定为枯草芽孢杆菌。采用枯草芽孢菌株进行发酵蔗渣中试,获得黄腐酸含量为17.2%,较工厂对照组黄腐酸含量(14.3%)提高了20%。

To enhance the capacity of producing fulvic acid（FA） from bagasse fermentation,microorganism strains were isolated and cultured from biohumic substances by determining the capacity of degrading cellulose at high temperature.The strains with aggressive growing and degrading were inoculated further to bagasse for its decomposing.Finally the optimized strain in degradation was selected and identified by 16sDNA,then applied eventually for an industrial trial on bagasse fermentation.The results showed that the majority of strains in isolated humus samples were dominated absolutely by Bacillus.Among those,the highest content of fulvic acid was observed in strain-E identified as Bacillus subtilis with 16sDNA.The FA content produced by Bacillus subtilis in industrial trial of bagasse fermentation was 17.2% that was 20% increased on the FA content produced by CK of the trial（14.3%）.