超临界流体萃取紫芝挥发油成分的GC-MS分析

GC-MS Analysis of the Essential Oil from Ultrafine-Powder of Ganoderma sinense by Supercritical CO_2 Fluid Extraction

为进一步研究紫芝挥发油的化学成分,采用超临界CO2流体萃取法从紫芝超细粉中提取分离挥发油,气相色谱-质谱联用分析其化学成分。从挥发油萃取物(1.47%,w/w)中检出46个峰(组分),鉴定了其中26个化合物(匹配度>83%),总相对含量占79.2%,主要成分有十六(烷)酸(18.88%)、(E)-十八碳-9-烯酸甲脂(18.74%)、(Z)-9-十八碳烯酸(24.33%)、(E)-2-癸烯醛(0.77%)、(E,E)-葵二烯醛(1.53%)、1,8,8-三甲基-2-(3-氧代环戊基)-三环[3.2.1.0(2,4)]辛烷-3,3-二腈(4.41%)、9-甲基-Z-1-十四碳-1-醇乙脂(1.20%)、(Z)-9-十八碳烯酸-2-羟基-1-羟甲基乙脂(1.95%)和E-11-十六烯-1-醇(2.38%)等。超临界流体萃取分离的紫芝挥发油成分及其相对含量有别于乙醚回流-水蒸气蒸馏法提取得到的挥发油成分。

Objective：Further to analysis chemical components in the essential oil（EO） of Ganoderma sinense.Methods：EO was extracted using Supercritical CO2 fluid extraction（SFE-CO2） technique from the ultrafine-powder of Ganoderma sinense,and detected by Gas chromatography-mass spectrometry（GC-MS）.Results： 46 compounds were checked out from the EO extract（with 1.47% of extract rate,w/w）,and 26 compounds（matching degree 83%） were identified representing 79.2% of the total EO,including n-Hexadecanoic acid,（E）-9-Octadecenoic acid methyl ester,（E）-9-Octadecenoic acid and（E,E）-2,4-Decadienal,9-Methyl-Z-10-tetradecen-1-ol acetate,1,8,8-trimethyl-2-（3-oxocyclo-pentenyl）-Tricyclo[3.2.1.0（2,4）]octane-3,3-dicarbonitrile,（Z）-9-Octadecenoic acid-2-hydroxy-1-（hydroxymethyl） ethyl ester and E-11-Hexadecen-1-ol and so on.Collusion： The results indicated that chemical components and their relative contents of the EO extracted by SFE-CO2 were different from that by steam distillation（SD） after ether refluxing.