活血丹子叶无性系建立的研究

Study on Establishment of Clone for Giechoma longituba

为保护资源和实现栽培,以活血丹的子叶为材料,采用组织培养的方法,进行了愈伤组织诱导与分化、试管苗生根、继代增殖、扦插和定植的研究,建立起无性系。结果表明:MS+KT 0.4 mg.L-1+2,4-D1.8mg.L-1是愈伤组织诱导培养和继代增殖培养的理想培养基;1/2MS+AgNO30.7 mg.L-1+ZT0.2mg.L-1+NAA 0.1mg.L-1是愈伤组织分化培养的理想培养基;用浓度为2.0mg.L-1的ABT2号溶液对不定芽进行48h处理、N6+IAA 0.2mg.L-1是不定芽生根培养的理想培养基;N6+ABT2号0.5mg.L-1+IAA 0.4mg.L-1是试管苗生根继代增殖培养的理想培养基;试管苗扦插成活率为96.3%,定植成活率为98.2%;定植的试管苗保持了活血丹的所有植物学性状。

In order to protect wild resources and realize cultivation,the cotyledons were used as materials to do the research on callus induction and differentiation,rooting and transplanting of tube seedlings by using tissue culture methods,finally established clone of Giechoma longituba.The results showed that the optimum medium for callus induction and differentiation was MS＋KT 0.4 mg·L-1 L＋2,4-D 1.8 mg·L-1 and 1/2MS＋AgNO3 0.7 mg·L-1＋ZT 0.2 mg·L-1＋NAA 0.1 mg·L-1,respectively.After dealt with ABT 2（concentration of 2.0 mg·L-1）for 48 h,the adventitious buds could be induced rooting in the medium of N6＋IAA0.2 mg·L-1.The ideal medium for rooting of the subculture was N6＋ABT 2 0.5 mg·L-1＋IAA 0.4 mg·L-1.The transplanting survival rate of tube seedlings was 96.3% and stable planting survival rate was 98.2%.Colonization of plantlets maintained for all biological traits of Giechoma longituba.