全叶延胡索愈伤组织诱导及快速繁殖的研究

The Study on Callus Induction and Rapid Propagationof Corydalis Repens

为保护野生资源，满足栽培需要，选择全叶延胡索有叶嫩茎为材料，进行了愈伤组织的诱导和分化，不定芽生根，试管苗生根继代增殖、移栽和定植的研究，建立起快速繁殖技术。结果证明：MS＋6-BA0．3mg／L＋2，4-D2．4mg／L是有叶嫩茎愈伤组织诱导培养和继代增殖培养的理想培养基；MS＋ZTO．1＋AgN031．0mg／L＋IAA0．1mg／L是愈伤组织分化培养的理想培养基；1／3MS＋ABT2号0．6mg／L＋IAA0．5mg／L是不定芽生根培养和试管苗生根继代增殖培养的理想培养基。无根茎试管苗移栽成活率为83．7％，有根茎试管苗移栽成活率为92．0％，试管苗定植成活率为98．6％，定植的试管苗保持了野生全叶延胡索的所有植物学性状。

In order to protect wild resources and meet the need of cultivation,the tender stems with leaves were used as mate- rials to do the research on callus induction and differentiation, rooting and transplanting of tube seedlings and so on, finally estab- lished the rapid propagation system of Corydalis repens. The results showed that the optimum medium for callus induction and sub- culture was MS ＋ 6 - BA0.3 mg/L ＋ 2,4 - D2.4mg/L; MS ＋ ZT0. 1 ＋ AgNO31.0 mg/L ＋ IAA 0.1 mg/L was the ideal medium for callus differentiation and the adventitious buds could be easily induced rooting in the medium of 1/3MS ＋ ABT2 0.6mg/L ＋ IAA0. 5mg/L. The transplanting survival rate of tube seedlings without rhizome was 83.7% and the transplanting survival rate of tube seedlings with rhizome was 92.0%. The stable planting Survival rate was 98.6%. Colonization of plantlets maintained for all biolog- ical traits of Corydalis repens.