Annexin-1与VEGF-C在食管鳞癌中的表达和临床意义

Expressions and significances of Annexin-1 and VEGF-C in esophageal squamous cell carcinoma

目的:检测膜联蛋白-1(Annexin-1)与血管内皮生长因子C(Vascular endothelial growth factorC,VEGF-C)在食管鳞状细胞癌及癌旁正常食管鳞状上皮中的表达差异。方法:应用免疫组化S-P方法检测80例食管鳞状细胞癌及其癌旁正常鳞状上皮中Annexin-1与VEGF-C的表达情况。结果:Annexin-1在癌旁正常食管鳞状上皮中67例(83.75%)呈阳性表达;在鳞状细胞癌中31例(38.75%)呈阳性表达,统计学处理差异有显著性(P<0.05)。食管鳞状细胞癌Annexin-1表达下降与患者的年龄、性别、肿瘤分化程度、浸润深度及淋巴结转移均无关(P>0.05)。VEGF-C在食管鳞状细胞癌中57例(71.25%)呈阳性表达;在食管癌旁正常食管鳞状上皮中37例(46.25%)呈阳性表达,统计学处理差异有显著性(P<0.05)。食管鳞状细胞癌VEGF-C表达升高与患者的年龄、性别、肿瘤分化程度及浸润深度无关(P>0.05),与淋巴结转移有关(P<0.05)。Annexin-1与VEGF-C蛋白表达呈负相关关系(r=-0.596,P<0.05)。结论:Annexin-1蛋白在食管鳞状细胞癌中有不同程度的丢失,而VEGF-C蛋白在食管鳞状细胞癌中有不同程度的表达上调,两者很可能成为食管癌早期诊断、治疗以及预测食管癌患者预后的具有重要应用价值的指标。

Objective To detect the expressions of Annexin-1 and vascular endothelial growth factor C （VEGF-C） in esophageal squamous cell carcinoma （ESCCs） and paraneoplastic normal squamous epithelium. Methods The expressions of Annexin-1 and VEGF-C in 80 cases of ESCCs and 80 cases of the corresponding paraneoplastie normal squamous epithelium were measured by immunohistochemistry S-P method. Results Annexin- I was positively expressed in 67 cases （83.75%） of paraneoplastic normal squamous epithelium, and in 31 cases （38.75%） of ESCCs, there existed significant difference between them （P〈O.05）. There were no significant correlations of the decreased expression of Annexin-1 with the age, sex, differentiation, the depth, of invasion, and lymph node metastasis （P 〉 0.05）. The VEGF-C was positively expressed in 57 cases （71.25%） of ESCCs, and in 37 cases （46.25%） of paraneoplastic normal squamous epithelium, there existed significant difference between them （P 〈 0.05）. The increased expression of VEGF-C was not correlate with the age, sex, differentiation, and the depth of invasion （P〉 0.05）, but was correlated with lymph node metastasis （P 〈 0.05）. The expression of Annexin-1 was negatively correlated with VEGF-C （r = -0.596, P 〈 0.05）, Conclusions The low expression of Annexin-1 and over expression of VEGF-C in the ESCCs indicate that they may be used in the early diagnosis, therapy, and prognosis in ESCCs.