广东女性人群μ受体基因118A→G突变对吗啡镇痛效果的影响

A118G single nucleotide polymorphism of the μ-opioid receptor affect morphine analgesia in cantongnese healthy volunteers

目的:研究μ受体基因(OPRM1)118A→G突变对吗啡镇痛效果的影响。方法:应用PCR-RFLP法测定广东49例健康女性人群OPRM1118bp处等位基因的基因型;根据基因型将受试者分为AA组、AG组、GG组,测定基础痛阈值和吗啡干预后的痛阈值。结果:各种基因型所占比例GG(8,16.3%)、AA(18,36.7%)、AG(23,47.0%)。各组受试者给予吗啡干预后痛阈值较基础痛阈明显升高(P<0.05);干预后痛阈与基础痛阈间的差值,GG组明显小于AA组(P<0.05)和AG组(P<0.05)。结论:发生OPRM1118A→G突的GG型基因女性携带者的吗啡镇痛效应明显弱于野生型(AA型)和杂合型(AG型)。

Objective In this study, we investigated whether A118G single nucleotide polymorphism of the p.-opioid receptor （OPRM1） affect morphine analgesia in healthy female volunteers. Methods Forty-nine healthy female volunteers of eantongnese were included in the study.The genotypes were determined by sequencing polymerase chain reaetionamplified DNA. The volunteers were divided into three groups according to OPRM1 genotypes （AA, AG, GG）, and their pain threshold was determined with transcutaneous electrical stimulation before and after the application of morphine. Results The percentage of three genotypes is AA 36.7% （18 cases ）, AG 47.0% （23 cases）,GG 16.3% （8 cases）respectively. The pain threshold after the application of morphine was markedly raised compared with before in each group. The changes in group GG was significantly lower compared with group AA or group AG. Conclusion Female carrying OPRM1 G118 homozygotes have a poorer response to morphine than A118 homozygotes or heterzygotes.