连续性肾脏替代治疗对严重脓毒症患者外周血T淋巴细胞免疫功能的影响

Effect of CRRT on immune function of peripheral blood T lymphocytes-mediated in patients with severe sepsis

目的:探讨连续性肾脏替代治疗(CRRT)对严重脓毒症患者外周血T淋巴细胞免疫功能的影响。方法:将85例严重脓毒症患者随机分成两组,对照组给予常规治疗,观察组在常规治疗基础上加CRRT治疗,观察两组疗效;治疗5d后,体外分离、培养外周血T淋巴细胞,采用MTT法检测治疗前后外周血T淋巴细胞增殖反应;采用ELISA法检测培养上清IL-2的分泌量。结果:观察组治愈率为81.4%,明显高于对照组59.5%(P<0.05);观察组较对照组T细胞增殖反应及IL-2表达量升高(P<0.05)。结论:CRRT治疗脓毒症疗效明显,并能有效改善严重脓毒症患者T淋巴细胞免疫功能。

Objective To investigate the effect of CRRT on immune function of peripheral blood T lymph0cytes in patients with severe sepsis. Methods Eighty-five patients with severe sepsis were randomly divided into two groups： the CRRT treatment group and the control group, the patients in the CRRT group were received routine therapy and CRRT. Peripheral blood T lymphoeytes were cultured and incubated with 20 μg/mL of phytohemagglutin （PHA） at 37℃ under 5% CO2. Cell proliferation was assessed by tetrazolium blue （MTT） assay 68 hours after PHA incubation. The IL-2 concentration in the supernatant fluid was determined by enzyme linked immunosorbent assay（ELISA）. Results The recovery rate of patients in the CRRT group was 81.4% which was higher than 59.5% in the control group. Proliferation activity of peripheral blood T lymphocytes and IL-2 level in CRRT group were significantly higher than those in the control group. Conclusion CRRT can improve immune function of T lymphocyte in patients with severe sepsis, contributing to the treatment effect of patients with severe sepsis.