三氧化二砷对小鼠大脑组织神经递质代谢酶基因及其受体基因表达谱的影响

Influence of Arsenic Trioxide on Gene Expression Profiles of Metabolic Enzymes and Receptors for Neurotransmitters in Cerebrum of Mice

[目的]研究亚慢性砷暴露对小鼠大脑组织神经递质代谢酶基因及其受体基因表达谱的影响。[方法]小鼠按体重随机分为3组,每组2只:4mg/L三氧化二砷(As2O3)染毒组、1mg/L As2O3染毒组和生理盐水对照组。染毒组小鼠饮用含As2O3自来水,对照组饮用0.9%生理盐水,各组小鼠每天消耗水量约20mL,均连续饮用60d,之后对其大脑组织进行基因芯片研究。[结果]与对照组比较,染砷组小鼠大脑组织的基因Th、Dbh、Tph1、Drd3、Drd4、Adra1a、Adra2a、Adra2b和Adrb3表达下调,而基因Htr1f、Htr4、Htr7表达上调。[结论]As2O3可能导致小鼠大脑组织部分神经递质合成和分解代谢酶基因表达下调,且干扰其部分受体基因的表达。

[ Objective ] To study the effect on gene expression profiles of metabolic enzymes and receptors for in cerebrum of mice by chronic exposure. [ Methods ] The rats were randomly divided into three groups ： 4mg/L arsenic trioxide （As2O3）, 1 mg/L As203, and the control groups. After 60 days of exposure to As2O3 through drinking water, expressions of target genes in cerebrum of mice were studied by genechip. [ Results ] Compared to the control group, the expressions of gene Th, Dbh, Tphl, Drd3, Drd4, Adrala, Adra2a, Adra2b, and Adrb3 of mouse cerebrum in two treated groups were statistically down-regulated, while the expressions of gene Htrlf Htr4, and Htr7 were up-regulated. [ Conclusion ] As2O3 may down-regulate the gene expressions of neurotransmitter synthesis and catabolism enzymes and interfere with the gene expressions of partial neurotransmitter receptors in mouse cerebrum.