3-乙酰基-4-乙酰氧基苯并噁唑-2-酮对四氯化碳致小鼠急性肝损伤的保护作用

Protective Effect of 3-Acetyl-4-acetoxy-2-benzoxazolone against Acute Liver Injury Induced by Carbon Tetrachloride in Mice

目的观察3-乙酰基-4-乙酰氧基苯并噁唑-2-酮(3-acetyl-4-acetoxy-2-benzoxazolone,TC-3)对四氯化碳所致小鼠急性肝损伤的保护作用,并探讨其疗效机制。方法采用腹腔注射体积分数0.15%四氯化碳制备小鼠急性肝损伤模型,3-乙酰基-4-乙酰氧基苯并噁唑-2-酮各剂量组灌胃给药,检测不同剂量的3-乙酰基-4-乙酰氧基苯并噁唑-2-酮对肝损伤小鼠血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶活性以及肝组织匀浆中超氧化物歧化酶活性、丙二醛和还原型谷胱甘肽含量的影响,同时光镜下观察肝组织的病理变化。结果 3-乙酰基-4-乙酰氧基苯并噁唑-2-酮能明显降低四氯化碳致急性肝损伤小鼠血清血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶的活性,降低肝匀浆丙二醛的含量,同时提高肝组织中超氧化物歧化酶、谷胱甘肽的活性,并能减轻四氯化碳对肝组织的病理损伤。结论 3-乙酰基-4-乙酰氧基苯并噁唑-2-酮对四氯化碳致小鼠急性肝损伤具有一定的保护作用,其保护机制可能与清除自由基、抗脂质过氧化作用有关。

OBJECTIVE To study the protective effect of 3-aeetyl- 4-aeetoxy-2-benzoxazolone on liver injury induced by carbon tetrachloride （ CCl4 ） in mice. METHODS The serum activities of ALT and AST and the levels of MDA, SOD and GSH in liver tissue were measured in the model of acute liver injury induced by intraperitoneal injection 0. 15% CCl4 and hepatic histological changes were observed by optical microscope. RESULTS Compared with control group, the serum ALT, AST and the liver tissue MDA activities were significantly lowered, the levels of SOD and GSH in liver tissue were increased obviously. Histological results showed that 3-ace- tyl- 4-acetoxy-2-benzoxazolone could ameliorate histological damages induced by CCl4 in mice. CONCLUSION 3-Aeetyl- 4-aeetoxy- 2-benzoxazolone exhibits protective effects on experimental hepatic injury.