异氟醚对PC12细胞株细胞周期与凋亡的影响

Effects of isoflurane on cell cycle and apoptosis in PC12 cells

目的探讨异氟醚对PCI2细胞株细胞周期与凋亡的影响。方法经神经生长因子孵育7d的神经元样PCI2细胞，培养于25crn2培养瓶，采用随机数字表法，将其随机分为2组（n=6）：对照组（c组）和异氟醚组（I组）。C组正常培养，I组用1．2％异氟醚处理12h。采用倒置相差光学显微镜观察细胞形态；收集细胞，采用流式细胞术测定细胞凋亡率、细胞周期、线粒体膜电位（MMP）及胞浆钙离子浓度（[Ca2＋]．）。结果与C组比较，I组G0／G1期细胞比例减少，S期和G2/M期细胞比例增加，细胞凋亡率增加，MMP降低，[Ca2＋]．增加（P〈0．05）。l组细胞形态发生损伤变化。结论1．2％异氟醚处理神经元样PCI2细胞12h，可异常启动细胞周期，致细胞发生凋亡。

Objective To investigate the effects of isoflurane on the cell cycle and apoptosis in PCI2 cells. Methods The neuronal PC12 cells were cultured for 7 d with nerve growth factor in vitro. The cells were cultured in 25 cm2 culture flask and randomly divided into 2 groups （n = 6 each） ： control group （group C） and isoflurane group （group I）. The neuronal PC12 cells were exposed to 1.2% isoflurane for 12 h in group I. The cell morphology was examined by light microscopy. The apoptotic rate, cell cycle progression, mitochondrial mem- brane potential （MMP） and intracellular Ca2. concentrations （ [ Ca2 ＋ ] i ） were assessed by flow eytometry. Results Compared with group C, the cell morphology was changed, the proportion of the cells in G0/G1 phase and MMP were signiilcantly decreased, and the proportion of the cells in G2/M phase, apoptotic rate and [ Ca2＋ ]i were sig- nificantly increased in group I （ P 〈 0.05） . Conclusion Exposure of PC12 cells to 1.2% isoflurane for 12 h can activate the cell cycle abnormally and result in cell apoptosis.