菌种添加量对生物预处理小麦秸秆厌氧发酵的影响

Effects of Supplement Dose of Microorganism in Biological Pretreatment of Wheat Straw on Its Characteristics of Anaerobic Digestion for Biogas Production

对小麦秸秆进行了氨-生物联合预处理,在实验室自制的小型厌氧发酵装置上,对预处理后的小麦秸秆进行了厌氧发酵制取沼气试验,探讨了氨-生物联合预处理中,菌种的添加量对小麦秸秆厌氧发酵产沼气的影响。结果表明,在氨预处理尿素溶液质量浓度为35 g/L,生物预处理pH值为4,黄孢原毛平革菌和里氏木霉的添加比例为1∶1(数量级为109)的条件下,小麦秸秆厌氧发酵过程中沼气总产气量最大,为7 968 mL,较空白组提高了23.11%。发酵过程终了pH值、VFA和甲烷的变化均在正常的范围内,甲烷最高体积分数为51.33%,较空白组提高了6.01%,整个发酵过程历时23 d。

Co-pretreatment of wheat straw by urea and microorganism was conducted. The effects of supplement dose of microorganism in biological pretreatment of wheat straw on its anaerobic digestion for biogas production were investigated by using a self-made anaerobic digestion device with pretreated wheat straw as raw materials. Results showed that the total yield of biogas reached the maximum of 7 968 mL, which was 23. 11% higher than the control, when the urea concentration, pH value of biological pretreatment and ratio of Phanerochaete chrysosporium to Trichoderma reesei added into the wheat straw were 35 g/L, 4, and 1： 1, respectively. The values of VFA, CH4 and pH value were located in normal range. The maximum CH4 content was 51.33% , which was 6.01% higher than that of control. The fermentation time was shortened to 23 d during the whole experiment.