HPLC法同时测定山玫胶囊中8个成分的含量

Simultaneous determination of eight components in Shanmei Capsules by HPLC

目的:建立同时测定山玫胶囊中没食子酸、绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷和槲皮素含量的高效液相色谱方法。方法:采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm,5μm),以0.5%甲酸水溶液(A)-乙腈(B)-甲醇(C)-四氢呋喃(D)为流动相梯度洗脱,流速1.0 mL.min-1,检测波长260 nm、参比波长360 nm(0～15 min),检测波长370 nm、参比波长430 nm(15～65 min),柱温30℃。结果:没食子酸、绿原酸、牡荆素葡萄糖苷、牡荆素鼠李糖苷、芦丁、牡荆素、金丝桃苷和槲皮素的线性范围分别为9.38～300,11.2～360,18.7～600,31.8～1020,1.31～42.0,7.50～240,9.00～288,0.563～18.0μg.mL-1;r分别为0.9999,0.9995,0.9999,0.9999,0.9998,0.9998,0.9998,0.9999。平均加样回收率(n=6)分别为99.5%(RSD=1.6%),99.0%(RSD=1.2%),99.8%(RSD=1.7%),99.8%(RSD=1.3%),100.5%(RSD=1.8%),99.6%(RSD=1.5%),99.9%(RSD=2.0%),100.6%(RSD=1.1%)。结论:该分析方法准确可靠,重复性好,为更好地控制山玫胶囊内在质量提供科学依据。

Objective： To develop an HPLC method for simultaneous determination of gallic acid, chlorogenic acid, glucosyl - vitexin, vitexin - 2＂ - O - rhamnoside, rutin, vitexin, hyperoside and quercetin in compound Shanmei Capsules. Methods： The separation was carried out on an Agilent ZORBAX SB -Cls column（4.6 mm 250 mm, 5 μm） with 0.5% formic acid（A） - acetonitrile（B） - methanol（C） - tetrahydrofuran（D） as the mobile phase in a gradient elution mode. The flow rate was 1.0 mL min-1 at 0 - 15 min,the detection wavelength was 260 nm with the reference wavelength of 360 nm; at 15 - 65 min, the detection wavelength was 370 nm,the reference wavelength was 430 nm. The column temperature was 30 ℃. Results： The linear ranges of gallic acid, chlorogenic acid, glu- cosyl - vitexin, vitexin - 2＂ - O - rhamnosid, rutin, vitexin, hyperoside and quercetin were 9.38 - 300 μg mL- 1 （ r = 0. 9999 ）, 11.2 - 360 μg mL -1 （ r = 0. 9995 ）, 18.7 - 600 μg mL- 1 （ r = 0. 9999 ）, 31.8 - 1020 μg mL - （r=0.9999）,1.31-42-0 μg mL-1（r=0.9998）,7.50-240 μg mE-l（r=0-9998）,9.00-288 μg mL-1 （ r = 0. 9998 ） ,0. 563 - 18.0 μg mL-1 （ r = 0.9999 ） , respectively. The average recovery rates of them were 99.5% （RSD = 1.6% ） ,99.0% （RSD = 1.2% ） ,99.8% （RSD = 1.7% ） ,99.8% （RSD = 1.3% ）, 100.5% （RSD = 1.8% ） ,99.6% （RSD = 1.5% ） ,99.9% （RSD =2.0% ） ,and 100.6% （RSD = 1. 1% ） , respectively. Conclusion： This method provides a scientific basis for the quality control of compound Shanmei Capsules.