罗布麻叶类药材中3个黄酮苷成分HPLC定量分析

HPLC quantitative analysis of three flavonoid glycosides in Folium Apocyni Veneti and Folium Poacyni

目的:建立HPLC法定量分析罗布麻叶类(红麻和白麻)药材中3种黄酮苷类成分。方法:采用Elite-ODS(5μm,150 mm×4.6 mm)色谱柱,以乙腈-水-四氢呋喃-冰醋酸(8∶81∶10∶1)为流动相,流速1.0 mL.min-1,检测波长258 nm,柱温30℃。结果:芦丁、异槲皮苷和槲皮苷线性范围分别为0.026～0.520,0.094～1.880,0.084～1.680μg;相关系数分别为0.9996,0.9997,0.9990。回收率(n=5)分别为97.5%,98.4%,97.7%;RSD分别为1.9%,1.8%,2.2%。药材红麻和白麻中黄酮苷类成分含量和分布存在差异。结论:所建立的方法快捷、灵敏、准确,可作为鉴别和评价罗布麻叶类药材的质量控制方法。

Objective： To establish an HPLC method for the determination of three flavonoid glycosides in Folium Apocyni Veneti and Folium Poacyni. Methods： The analysis was performed on an Elite - ODS（5 μm, 150 mm× 4.6 mm） column with the mobile phase consisted of acetonitrile - water - tetrahydrofuran - acetic acid（ 8：81 ： 10：1 ） at a flow rate of 1.0 mL min-1, the detection wavelength was 258 nm and the column temperature was 30 ℃. Results： The linear ranges of rutin, isoquercitrin and quercitroside were 0.026 - 0.520, 0.094 - 1. 880 and 0.084 - 1. 680 μg with the correlation coefficients of 0. 9996 ,0. 9997 and 0.9990, respectively. The average recoveries （ n = 5 ） were 97.5%, 98.4%, 97.7% with RSDs of 1.9%, 1.8%, 2.2%. Difference in the distributions and contents of isoquercitrin from two herb medicines was also shown from HPLC analysis. Conclusion： This method is simple, sen- sitive and rapid, which can be used for the quality control of Folium Apocyni Veneti and Folium Poaeyni.