Prohibitin rs6917 C>T基因多态性与潮汕女性乳腺癌易感性的关系研究

Association between rs6917 C>T polymorphism of prohibitin and breast-cancer susceptibility in Chaoshan Women

目的研究潮汕汉族女性人群中Prohibitin（PHB）基因rs6917 C〉T单核苷酸多态性（single nucleotide polymor-phism,SNP）与乳腺癌遗传易感性的关系。方法采用病例-对照研究,于2009年7-12月收集汕大医学院附属肿瘤医院231例乳腺癌住院患者和169例正常对照女性的外周血,提取全血基因组DNA,以高分辨率熔解曲线（highresolution mehing,HRM）法检测PHB基因rs6917位点基因型,计算各种基因型的乳腺癌发生风险及其95%可信区间。结果潮汕地区女性人群PHB rs6917存在两种基因型CC和TC,在乳腺癌组的频率分别为74.46%（C/C）、25.54%（T/C）,在正常人群的频率分别为72.78%（C/C）和27.22%（T/C）。经统计学分析,T/C基因型携带者与C/C基因型携带者在乳腺癌发病风险上并无显著性差异（OR=1.090,95%CI=0.695~1.709,P=0.706）。结论未发现PHB基因rs6917位点SNP与潮汕汉族女性乳腺癌易感性具有相关性。

Objective To investigate the association between rs6917 C〉T single nucleotide polymorphism （SNP） of prohibitin and risk of breast cancer in Chaoshan women. Methods A case-control study was used. From July 2009 to December 2009, 23I breast-cancer patients hospitalized in cancer hospital of Shantou university medical college and 169 healthy controls were enrolled into this study. The genomic DNA was extracted from whole blood cells. Genotypes were determined determined by the high resolution melting （HRM） method. Results The PHB rs6917 C〉T genotype frequencies were 74.46% （C/C）, 25.54% （T/C） in the breast cancer group and 72.78% （C/C）, 27.22% （T/C） in the control group respectively. The genotype frequencies in patients and controls did not differ overall in our sample （odds ratio =1.090, 95%CI=0.695- 1.709, P = 0.706）. Conclusion Our results do not lend support to the hypothesis that PHB rs6917 C〉T polymorphism contributes to risk of breast cancer in Chaoshan women.