摘要
目的探讨维生素D受体(VDR)基因FokI位点多态性与1型糖尿病(T1DM)的关系。方法研究纳入T1DM患者241例和正常对照组380例。VDR基因FokI位点基因型采用聚合酶链反应-限制性片断长度多态(PCR-RFLP)法检测。结果 T1DM组VDR基因FokI基因型分布和F/f等位基因频率与对照组比较,两者间的差异无统计学意义(P>0.05)。在T1DM组中,Ff基因型组与ff基因型组的空腹-C肽(FCP)和餐后C-肽(PCP)均低于FF基因型组,差异具有统计学意义(P<0.05)。结论 VDR基因FokI多态性与T1DM遗传易感性不相关。T1DM患者中ff基因型与Ff基因型组FCP和PCP呈低水平,提示VDR基因FokI基因型可能与胰岛细胞功能相关。
Objective To explore the association between the VDR gene Fok I polymorphism and type 1 diabetes （T1DM）.Methods Two hundred and forty-one T1DM patients and 380 healthy subjects were recruited in the research.Length poly morphism polymerase chain reaction （RFLP-PCR） was performed to identify VDR gene Fok I genotypes.Results No differences were observed between T1DM and controls in VDR Fok I genotype distribution and allele frequencies （P 0.05）.In T1DM group,however,lower levels of fasting C-peptide （FCP） and postprandial C-peptide （PCP） were found in Ff and ff genotypes than FF genotype,the differences were statistical significance （P 0.05）.Conclusion There is no association between the VDR gene Fok I genotype and T1DM.VDR gene Fok I Ff and ff genotypes showe lower FCP and PCP levels,which suggest the VDR gene Fok I genotype is associated with islet cell function in T1DM.
出处
《中国当代医药》
2012年第21期5-7,共3页
China Modern Medicine
基金
国家自然科学基金(81100573)
