何首乌组培快繁培养基的优化

Optimization on Medium for Tissue Culture and Rapid Propagation of Polygonum multiflorum

为筛选出适宜何首乌组织培养的培养基,以带腋芽茎段为外殖体,采用完全随机区组试验设计,进行不同浓度激素(6-BA,NAA,IBA,6-BA+NAA,6-BA+IBA,多效唑)的培养基对何首乌产生丛生芽及不定根的诱导作用研究。结果表明:一定浓度范围内6-BA对诱导何首乌产生丛生芽具有促进作用,NAA和IBA对丛生芽的诱导具有抑制作用,适宜何首乌丛生芽增殖的培养基为MS+6-BA 1.0mg/L,增殖系数达11.43;多效唑对不定根的诱导具有促进作用,最适生根的培养基为1/2MS+多效唑0.5mg/L,生根率为100%,平均生根条数为10.42条/株。

To screen the optimum culture condition for the tissue culture of Polygonum multiflorum,the stem with axillary bud of P.multiflorum.was used as explants in the experiment with the completely random block design and the media inducing multiple shoots and adventitious roots contained different concentrations of 6-BA,NAA,IBA,6-BA and NAA,6-BA,IBA and paclobutrazol）.The results showed that 6-BA could promote to induce multiple shoots,and NAA,IBA inhibit the induction of buds in a certain concentration range.Moreover,the optimum medium for multiplication and differentiation was MS＋6-BA 1.0 mg/L and its multiplication coefficient reached 11.43.Paclobutrazol had a promotion on the adventitious root induction,the optimum rooting medium for 100% rooting rate was 1/2 MS＋ paclobutrazol 0.5 mg/L,and average rooting number was 10.42 per plant.