不同溶剂和提取方法对红花提取物抗氧化活性的影响

Effects of Different Solvents and Extraction Methods on Antioxidant Activity of Extracts from Safflower

为了提高红花中的总酚提取率,评价其抗氧化活性,以水、70%乙醇、70%酸性乙醇、正丁醇、乙酸乙酯和正己烷为溶剂,采用冷浸提取、超声提取、回流提取和ASE快速溶剂萃取法对红花进行提取,采用Folin-Ciocalteu方法,DPPH抗氧化活性体外评价体系测定红花不同提取物的抗氧化活性。结果表明:4种提取方法中,回流提取总多酚含量最高,其余依次为超声提取、ASE提取和冷浸提取;6种不同溶剂提取总多酚含量效果排序为70%酸性乙醇>70%乙醇>水>正丁醇>乙酸乙酯>正己烷。结论:各提取方法中,70%酸性乙醇回流法提取物总多酚含量最高,抗氧化能力最强。

To enhance the extraction efficiency of total polyphenol from safflower,evaluate the antioxidant activity,taking water,70% alcohol,70% acidic alcohol,70% normal butanol,ethyl acetate and hexane as solvents,the total polyphenols were extracted from safflower by different methods,including quench,ultrasonic wave,reflux and ASE.And the antioxidant activity was evaluated by DPPH assay.The results showed that the total phenolic content（TPCs） extracted by reflux method was the highest,followed by ultrasonic wave method,ASE and quench;The extraction efficiency order of the six various solvents was 70% acidic ethanol70% alcoholwaternormal butanolethyl acetatehexane.Conclusion： The 70% acidic ethanol reflux method showed the highest TPCs,and had the strongest antioxidant activity.