茶花凤仙瓶苗开花诱导机理研究

Studies on in Vitro Flowering Technology of Impatiens balsamina

以茶花凤仙种子为外植体,获得无菌苗并进行增殖、壮苗培养和瓶内开花实验。结果表明:茶花凤仙的种子用1 g/L的氯化汞消毒9 min效果最好,成活率达到90%;采用正交试验设计方法筛选出无菌苗最佳增殖培养基配方为:MS+6-BA 0.6 mg/L+NAA 0.3 mg/L+蔗糖20 g/L+琼脂10 g/L,增殖系数可达3.6;对茶花凤仙的壮苗情况来看,PP333的最佳质量浓度为0.75 mg/L,采用的壮苗培养基配方为:MS+6-BA 0.6 mg/L+NAA 0.3 mg/L+PP333 0.75 mg/L+蔗糖20 g/L+琼脂10 g/L;经过壮苗后的植株在MS(1/3 NH4NO3)+6-BA0.6 mg/L+NAA 0.3 mg/L+PP333 0.75 mg/L培养基上培养,开花率最好,达58%。采用组织培养方法获得无菌苗,并通过芽的增殖与壮苗培养,诱导其在瓶内开花,可为其优良品种的开发利用和快速繁殖提供技术支持。

Seeds of Impatiens balsamina were used as explants , seedlings obtained were used for proliferation, strengthening cultivation and in vitro flowering. It was showed that seeds of Impatiens balsamina sterilized with 0.1% HgC12 for 9 minutes achieved the highest survival rate （90%）. The best medium for plantlet proliferation screened out by orthogonal experimental design method was ： MS ＋ NAA O. 3 mg/L ＋ 6-BA O. 6 mg/ L ＋ sucrose 20 g/L ＋ agar 10 g/L, the multiplication coefficient was up to 3.6. The best PP333 concentration was 0.75 mg/L for Impatiens balsamina and strengthening cultivation medium was MS ＋ 6-BA 0.6 mg/L ＋ PP333 0.75 mg/L ＋ NAA 0.3 mg/L ＋ sucrose 20 g/L ＋ agar 10 g/L. The modified MS medium （ 1/3 of NH4NO3 content） added with 6-BA O. 6 mg/L, NAA 0.3 mg/L and PP333 O. 75 mg/L was found to be the best medium for in vitro flowering , with flowering rate up to 58%.