球孢白僵菌对浙江双栉蝠蛾幼虫的致病力研究

A Study of the Virulence of Beauveria bassiana Strains against Bipectilus zhejiangensis Wang Larvae

应用8株球孢白僵菌[Beauveria bassiana(Bals.)Vuill]菌株分别对浙江双栉蝠蛾幼虫进行致病力测定,结果表明:以浓度(1.0±0.5)×106孢子/mL的孢子悬液接种12 d后,B3菌株对该虫有较强的致病力,其校正死亡率、僵虫率分别为(90.71±2.16)%和(87.07±0.95)%,致死中时(LT50)最短(6.20 d),且具较高的僵虫虫体含孢量(5.75×1010孢子/虫),该菌株对幼虫致死效应较强的时间在第6至第8 d。接菌后在第5天和第6天,B3菌株对该虫的LC50分别为1.35×108孢子/mL和1.23×106孢子/mL。B3菌株有较大的开发应用价值,B187菌株也有一定的应用潜力。

8 strains of B. bassiana were used to test for pathogenicity against B. zhejiangensis larvae. The results indicated that the strain B3 had higher pathogenicity. After inoculating strain B3 with （ 1.0 ± 0.5 ） × 106 conidia mL for 12 days, the correct mortality and the cadaver rate of larvae were （90.71 ±2.16）% and （87.07 ± 0.95 ）% respectively, and its median lethal time （LTso） was the shortest （6.20 days）. The average conidia individual of cadaver was significantly higher with 5.75 ×1010 conidia per larva. The stronger lethal time of the strain B3 against larvae was the 6th day to 8th day. The median lethal concentration （LC50） of the 5th and 6th day to larvae were determined at 1.35 × 108 conidia perlarva and 1.23 × 106 conidia perlarva respectively. It suggests that the strain B3 has great development application value, and strain B187 has certain application potential.