福建省商业猪种MUC13、IGF2和RYR1基因主效位点的遗传变异分析

Genetic Variation of MUC13,IGF2 and RYR1 Causative Mutations in Commercial Pig Breeds in Fujian Province

选择决定新生仔猪ETEC F4ac腹泻抗性的黏附素13(MUC13)基因,影响生长速度和背膘厚的IGF2内含子3 g.3072G>A,决定氟烷敏感性的RYR1 c.1843C>T等3个对养猪生产具有显著影响的基因位点,采用PCR-RFLP或PCR-SNaPshot方法,对福建省6家大型种猪场的杜洛克、长白、大白核心育种群进行基因型判定。结果表明:杜洛克、长白、大白的MUC13有利等位基因频率分别为0.892、0.643、0.638,IGF2有利等位基因频率分别为0.933、0.742、0.826,RYR1有利基因型频率很高,分别为:0.896、0.982、0.968。RYR1、IGF2与MUC13的有利等位基因型组合个体比例在各品种中差异较大,以杜洛克最高(65.4%),大白其次(28.4%),长白最低(19.9%)。因此,与常规育种技术相结合,需经过多世代选育才能将此3个主效位点的有利等位基因在受试种群中选育纯合。

MUC13 governs susceptibility / resistance to ETEC F4ac diarrhea in pighlets. IGF2 intron 3 g. 3072G 〉 A is a causal variant affecting muscle growth and backfat thickness. RYR1 c. 1843C 〉 T is responsible for malignant hyperthermia in pigs. In this study, PCR-RFLP or PCR-SNAPshot assay was used to genotype animals from nucleus pig populations in Fujian Province for the3 causal variants. The results showed that the favorable allele frequencies of MUC13 were O. 892, 0. 643 and 0. 638 in duroc, landrace and large white, respectively ; and those of IGF2 were O. 933, 0. 742 and O. 826 in the three commercial breeds, respectively. Much higher favorable allele frequencies of RYR1 were observed with 0.896, 0. 982 and 0. 968 seperately in the three breeds. Animals with favorable allele combination of RYR1, IGF2 and MUC13 were most prevalentin Duroc with a frequency of 65.4% while 28.4% in landrace and the lowest 19.9% in large white. Therefore, favorable alleles could be fixed in the tested breeds by marker-assisted selection through mutigenerations in combination of routine breeding techniques.