酶法辅助提取迷迭香中三萜酸的工艺研究

Study on Extraction Process of Triterpene Acids form Rosemary

为探讨迷迭香中三萜酸的最佳提取工艺条件,采用纤维素酶法,通过单因素试验和正交设计对迷迭香中三萜酸的提取工艺进行研究。酶解法提取迷迭香中三萜酸的最佳条件为:加酶量6.5 mg/g,酶解时间为2.5 h,酶解温度45℃,体系pH值6.5。在此条件下,所得迷迭香中三萜酸干质量为4.58 g。纤维素酶能有效地破坏细胞壁,加速有效物质的释放,纤维素酶酶解条件温和,工艺简单,值得推广应用。

To explore the optimum conditions for extraction of Triterpenoid acid in rosemary. Use ot cellulose enzyme, to research the extraction process of triterpenoid acid from rosemary by single factor experiments and orthogonal design. The best conditions for enzymatic extraction of triterpenoid acids from rosemary ： the amount of enzyme 6, 5 mg/g,hydrolysis time 2.5 h,reaetion temperature 45 ℃, pH value 6.5. Under these Conditions, the proceeds of triterpene acid dry weight were 4.58 g. Cellulase could effectively destroy the cell walls, to accelerate the release of active substances. The hydrolysis conditions of cellulase are mild, the process is simple, and worthy of wide application.