海南地区剑叶龙血树种质资源遗传多样性分析

Genetic diversity of Dracaena cochinchinensis germplasm resources in Hainan province

利用ISSR标记法对海南省野生剑叶龙血树资源进行多样性分析，从100条ISSR引物中选取10条多态性好、重复性高、谱带清晰的引物，对105份样品DNA进行扩增，共得到116条谱带，其中多样性谱带有104条。品种间遗传相似系数在O．6442～0．994之间，平均为0．8011，表明剑叶龙血树遗传基础相对较狭窄。利用UPGMA聚类分析，将7个样地的居群分为1个大类群和1个小类群，类群与地理分布密切相关。

Diversity of Hainan wild Dracaena cochinchinensis resourse was analyzed by ISSR markers, 10 primer pairs were selected from 100 ISSR primers as a result of better polymorphism, high repeatability, clear strips. A total of 116 bands was obtained, including 104 polymorphic bands. The range of GS was 0.6442-0.994, averaged 0.8011, which indicated a narrow genetic basis of D. cochinchinensis. Groups from 7 sample plot were divided into a large group and a small group, clustered by UPGMA method. Close relationship was found between the division of groups and local sources.