HPLC测定黄芩中6个成分的含量

Determination of Six Constituents in Scutellaria Baicalensis Georgi by HPLC

目的建立高效液相色谱法对黄芩中6个成分（黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、白杨素、千层纸素A）的含量分析方法。方法采用AgilentZORBAXSB—C18（250mm×4．6mm，5μm）色谱柱，以乙腈（A）-0．3％磷酸三乙胺（B）为流动相，梯度洗脱，检测波长为275nm，流速1．0mL．min-1，柱温30℃。结果黄芩中的6个成分黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、白杨素、千层纸素A进样量分别在0．4167-4．167μg（r=0．99921、0．07815～0．7815μg（r=0．99951、0．05321～0．5321μg（r=0．9993）、0．01636-0．1636pg（F0．9993）、0．001160-0．01160μg（r=0．9991）、0．01046-0．1046μg（r=0．9994）范围内呈良好线性关系；平均回收率（n=5）分别为99．4％、100．1％、99．9％、100．2％、100．4％、100．1％，RSD分别为1．9％、2．2％、2．3％、1．6％、2．2％、2．1％。结论该方法准确可靠、重复性好，为黄芩质量综合评价提供了科学依据。

OBJECTIVE To develop an HPLC method for determination of six indicative components（baicalin, wogonoside, baicalein, wogonin, chrysin, oroxylin-A） in Scutellaria baicalensis Georgi. METHODS Agilent ZORBAX SB-C18（250 mmx4.6 mm, 5 μm）was adopted; the mobile phase was acetonitrile（A）-0.3% phosphoric acid-triethylamine （B） with gradient elution, and the detection wavelength was set at 275 nm, and flow rate was 1.0 mL min-1 with the column temperature of 30℃. RESULTS The content of six elements in Scutellaria baicalensis Georgi was stable. The method had a good linearity in the ranges of 0.416 7-4.167 μg（r=0.999 2） for baicalin, 0.078 15-0.781 5 μg（r=0.999 5） for wogonoside, 0.053 21-0.532 1 μg（r=0.999 3） for baicalein, 0.016 36-0.163 6 μg（t=0.999 3） for wogonin, 0.001 160-0.011 60 μg（r=0.999 1） for chrysin, 0.010 46-0.104 6 μg（r=0.999 4） for oroxylin-A. The average recoveris（n=5） were 99.4%,100.1%, 99.9%, 100.2%, 100.4%, 100.1%, respectively with RSDs of the recoveries were 1.9%, 2.2%, 2.3%, 1.6%, 2.2%, 2.1%, respectively. CONCLUSION This method is acurate, simple and practical,and may be use to control quality of Scutellaria baicalensis Georgi.