高产漆酶平菇的筛选及其在降解黄曲霉毒素B_1中的应用

SCREENING HIGH-PRODUCTION LACCASE OF PLEUROTUS OSTREATUS AND DEGRADATION OF AFB_1 BY PLEUROTUS OSTREATUS LACCASE

通过选择培养基平板培养法和液体发酵培养法筛选得到2株高产漆酶的平菇菌株P1和P2,并对平菇菌株产漆酶的培养基进行筛选,得到产漆酶的最适培养基为最低盐MSM培养基。菌株P1不仅产漆酶能力最高,而且降解黄曲霉毒素的能力也最好。在MSM培养基中培养10d时,产漆酶量高达769.44U/L,在800μl的反应体系中,790μl粗酶液可以将1000ng黄曲霉毒素B1降解到222.62ng,降解率为77.74%,并且平菇粗酶液降解黄曲霉毒素B1的能力与其中漆酶的含量呈一定的正相关性。

Two high laccase-production strains Pleurotus ostreatus P1 and P2 were selected by selecting agar plates and liquid culture.MSM medium was the most suitable liquid medium for laccase-production.The best strain for laccase-producing and AFB1-degradating was Pleurotus ostreatus strain P1.The laccase activity could be 769.44U/L when it was cultured 10 days.1000ng AFB1 could be degradated to 222.62ng by 790μl crude enzyme solution and the degradation rate of AFB1 was 77.74%.There were positive corvelootions between AFB1-degradating and laccase actitity.