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褪黑素对大鼠光气吸入致肺损伤的保护作用 被引量:1

Protective effect of melatonin in rats with phosgene-induced lung injury
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摘要 目的探讨褪黑素(MT)对光气致大鼠肺损伤的抗氧化保护作用及其可能机制。方法50只SD雄性大鼠随机分为光气染毒组、空气对照组、MT处理组、地塞米松处理组(DX处理组)和阴性对照组,每组10只,除空气对照组外吸入8.33mg/L光气,染毒5rain,于染毒后1h分别从尾静脉注入MT(10mg/kg)、地塞米松(2.5mg/kg)、l%乙醇生理盐水(1ml/kg),于6h后处死动物,测定肺组织湿干比,支气管肺泡灌洗液中总蛋白含量及中性粒细胞计数以及肺匀浆中丙二醛(MDA)含量,超氧化物歧化酶(SOD)及髓过氧化物酶(MPO)活力;光学显微镜下观察肺组织病理;蛋白质印迹(Westernblot)法测定肺组织中诱导型一氧化氮合酶(iNOS)和核转录因子(NF.xB)的蛋白含量。结果与空对照气组比较,光气染毒组肺湿干比、BALF中中性粒细胞计数及蛋白含量均升高,差异有统计学意义(P〈0.01)。光气染毒组大鼠肺组织中的MDA含量、MPO活力较空气对照组明显升高,差异有统计学意义(P〈0.05)。与光气染毒组比较,MT处理组MDA含量和MPO活力均降低,SOD活力升高,差异有统计学意义(P〈0.01)。与光气染毒组比较,MT处理组和DX处理组iNOS及NF—xB蛋白质表达均降低,差异有统计学意义(P〈0.01)。结论MT对光气致肺损伤有保护作用,其机制可能与清除自由基抗氧化及抑制iNOS、NF.xB的表达有关。 Objective To investigate the antioxidant effect of melatonin (MT) in the rats with phosgene-induced lung injury and its possible mechanism. Methods Fifty male SD rats were equally randomized into phosgene exposure group, air control group, MT treatment group, dexamethasone (DX) treatment group, and negative control group. All groups except the air control group were exposed to 8.33 mg/L phosgene for 5 min, and the MT treatment group, DX treatment group, and negative control group were injected with MT (10 mg/kg), DX (2.5 mg/kg), and 1% ethanol saline (1 ml/kg), respectively, via the caudal vein 1 hour after exposure. The rats were sacrificed 6 h later. Then, the wet/dry ratio of the lung, the total protein content and neutrophil count in bronchoalveolar lavage fluid (BALF), and the malonaldehyde (MDA) content and superoxide dismutase (SOD) and myeloperoxidase (MPO) activities in lung homogenate were measured; pathological observation was made on the lung tissue under an optical microscope; the protein expression of inducible nitric oxide synthase (iNOS) and NF-xB in the lung tissue was measured by Western blot. Results Compared with the air control group, the phosgene exposure group showed significantly increased wet/dry ratio of the lung and total protein content and neutrophil count in BALF (P〈0.01) as well as significantly increased MDA content and MPO activity in the lung tissue (P〈0.05). Compared with the phosgene exposure group, the MT treatment group showed significantly decreased MDA content and MPO activity and significantly increased SOD activity (P〈0.01), and the MT treatment group and DX treatment group showed significantly decreased protein expression of iNOS and NF-KB (P〈0.01). Conclusion MT has protective effect in phosgene-induced lung injury, and its protective mechanism may be associated with scavenging free radicals and inhibiting expression of iNOS and NF-KB.
出处 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2012年第11期834-838,共5页 Chinese Journal of Industrial Hygiene and Occupational Diseases
基金 国家自然基金青年基金(81101412) 金山医院院课题(2010-7)
关键词 光气 褪黑素 丙二醛 超氧化物歧化酶 呼吸窘迫综合征 Phosgene Melatonin Malondialdehyde Superoxide dismutase Respiratory distresssyndrome
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