人参总皂苷对放射所致骨髓造血细胞衰老的保护作用

Effect of total saponins of Panax ginseng on irradiation-induced bone marrow hematopoietic cell injury and senescence

目的探讨人参总皂苷(total saponins of panax ginseng,TSPG)抗放射(6.5 Gy)所致骨髓造血细胞损伤衰老的作用。方法 40只雄性C57BL/6小鼠分为4组(每组10只):对照组、放射(irradiation,IR)组、IR+T50(TSPG 50 mg/kg)组、IR+T200(TSPG 200 mg/kg)组。先给药3 d,然后照射,照射后继续给药7 d,检测外周血的变化情况,计数各组骨髓单个核细胞数、骨髓造血细胞集落生成单位(CFU)的数量,观察形态变化;流式细胞术检测周期变化;回收CFU细胞进行β-半乳糖苷酶衰老(SA-β-gal)染色和细胞免疫荧光分析p16Ink4a蛋白的表达水平。结果与IR组比较,给药处理后,IR+T200组的白细胞数增加至(2.19±0.87)(P<0.05),但各组之间红细胞与血小板均无明显变化(P>0.05);IR+T200组的单个核细胞数及集落个数均明显增加(P<0.05);骨髓造血细胞周期G1期细胞比例随药物浓度增加有减少的趋势;SA-β-gal染色和p16Ink4a细胞免疫荧光染色均显示,IR+T200组的CFU中细胞阳性率明显降低(P<0.05)。结论合适剂量的TSPG(200 mg/kg)对放射引起的造血细胞损伤衰老有保护作用。

Objective To investigate the effect of total saponins of Panax ginseng（TSPG） on irradiation-induced bone marrow hematopoietic cell injury and senescence.Methods Forty male C57BL/6 mice were divided into four groups including a control group,an irradiation（IR） group,an IR＋T50 group（TSPG 50 mg/kg） and an IR＋T200 group（TSPG 200 mg/kg）.The mice were pretreated with TSPG for 3 days,then were irradiated by 6.5 Gy X-ray,and were further treated with TSPG for 7 days.Blood routine examination,bone marrow mononuclear cell（BM-MNC） counting and hematopoietic cell colony forming unit（CFU） counting were performed.The cell cycle of BM-MNC was determined by flow cytometry,and the senescence of cells from CFU was evaluated by senescence-associated β-galactosidase（SA-β-gal） staining and the expression of p16Ink4a protein.Results Compared with the IR group,the numbers of white blood cell（WBC）（2.19±0.87）,BM-MNC and CFU significantly increased in the IR＋T200 group（P0.05）,but the red blood cells and platelets showed no significant changes.The numbers of the cells at G1 phase decreased in a dose-dependent manner,and the cells from the CFU of the IR＋T200 group exhibited a decreased expression of SA-β-gal and p16Ink4a（P0.05）.Conclusion TSPG 200 mg/kg may alleviate irradiation-induced bone marrow hematopoietic cell injury and senescence.