摘要
目的筛选新的能够识别红内期约氏疟原虫17XL的TLRs。方法扩增并克隆Balb/c小鼠TLR1,TLR3,TLR5,TLR6,TLR7,TLR9,TLR11全长编码序列,并克隆至表达载体pcDNA3.1(+);搭建活化TLRs的NF-κB和IFN-β报告基因检测平台,并利用该平台系统筛选能够识别约氏疟原虫致死株17XL红内期超声刺激物的新TLRs。结果 NF-κB和IFN-β报告基因实验结果发现,红内期超声刺激物能够活化TLR2,TLR4,TLR9,但不能通过TLR3活化IFN-β,以及通过TLR5,TLR7,TLR9,TLR11活化NF-κB。结论除了能被TLR2(TLR2/TLR1,TLR2/TLR6),TLR4和TLR9识别外,17XL疟原虫红内期超声刺激物并不能被其他已知TLRs(TLR3,TLR5,TLR7,TLR11)所识别。
To identify a novel TLRs recognizing the components of Plasmodium Yoelii 17XL erythrocytic stage, We firstly amplified the full sequences coding TLR1, TLR3, TLR5, TLR6, TLR7, TLR9 and TLRll from mouse splenocytes, and cloned them into pcDNA3.1(+) expression vector. Then we established a platform to screen the TLRs agonists by measuring the activation level of NF-κB and IFN-β. By using this platform, we systematically screened the potential TLRs recognizing the ultrasonic product of Plasmodium Yoelii 17XL erythrocytic stage. We found that the ultrasonic product eould activate TLR2, TLR4 and TLR9, but not other known TLRs.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2012年第12期1036-1039,共4页
Immunological Journal
基金
国家自然科学基金面上项目(30972773)