十溴联苯醚孕期暴露对雌性子代大鼠生殖发育的影响

Effects of prenatal exposure to brominated diphenyl ethers-209 on reproductive development of female offspring rats

目的观察十溴联苯醚(BDE-209)孕期暴露对雌性子代大鼠生殖发育的影响。方法将24只SD孕鼠随机分为BDE-209低剂量(100 mg·kg-1·d-1)组、BDE-209中剂量(300 mg·kg-1·d-1)组、BDE-209高剂量(900 mg·kg-1·d-1)组和对照组(玉米油),每组6只。在雌性子鼠出生后第4天(PND4)、PND10、PND16、PND21、PND30和PND40时测量体质量、身长和尾长;在PND16和PND21时测量肛殖距;分别于PND21和PND40时处死1只雌性子鼠检测血清雌二醇水平;PND40时计算卵巢、肝脏和肾脏脏器系数,观察卵巢质量及病理变化。结果 BDE-209高剂量组雌性子鼠体质量、身长和尾长在部分生长发育时期明显低于对照组(P<0.05)。PND21时,BDE-209低剂量组雌性子鼠肛殖距明显大于对照组(P<0.05);PND40时,BDE-209中剂量组次级卵泡数量明显多于对照组(P<0.05);PND21和PND40时,各组雌性子鼠血清雌二醇水平比较,差异均无统计学意义(P>0.05)。结论 BDE-209孕期暴露可影响雌性子代大鼠的生殖发育。

Objective To investigate the effects of brominated diphenyl ethers-209 （BDE-209） on the reproductive development of female offspring rats. Methods Twenty-four pregnant SD rats were randomly divided into low-dose BDE- 209 group （exposure to 100 mg·kg^（-1）·d^（-1） BDE-209）, medium-dose BDE-209 group （exposure to 300 rag. kg^（-1）·d^（-1） BDE- 209）, high-dose BDE-209 group （exposure to 900 mg·kg^（-1） ·d^（-1） BDE-209） and control group （exposure to corn oil）, with 6 rats in each group. The body weight, height and tail length were obtained on postnatal day （PND） 4, PND10, PND16, PND21, PND30 and PND40, and anogenital distance was measured on PND16 and PND21 in female offspring rats. Serum estradiol levels were determined on PND21 and PND40 after sacrifice of 1 female offspring rat respectively. Organ coefficients of ovary, liver and kidney were calculated on PND40, and the weight and pathological changes of ovary were observed. Results The body weight, height and tail length in high-dose BDE-209 group were significantly lower than those in control group in part of the development period （P 〈 0.05）. The anogenital distance in low-dose BDE-209 group was significantly longer than that in control group on PND21 （P 〈 0.05）, the number of secondary follicles in medium-dose BDE-209 group was significantly larger than that in control group on PND40 （P 〈 0.05）, and there was no significant difference in serum estradiol levels among groups on PND21 and PND40 （P 〉 0.05）. Conclusion Exposure to BDE-209 during pregnancy can impair the reproductive development of female offspring rats.