DNA甲基转移酶在肝细胞癌中的表达及其临床意义

Expression and clinical significance of DNA methyltransferases in hepatocellular carcinoma

目的探讨DNA甲基转移酶1（DNMTl）、3a（DNMT3a）和3b（DNMT3b）在肝细胞癌（HCC）中的表达及其意义。方法应用免疫组织化学检测47例HCC癌组织、42例HCC癌旁肝组织及12例正常肝组织中DNMTl、DNMT3a和DNMT3b的表达情况，分析三者与临床病理特征的关系。结果DNMTl、DNMT3a和DNMT3b在HCC中阳性表达率分别为80．9％、68．1％和78．7％，在癌旁组织内分别为50．0％、52．4％和57．1％，均明显高于正常肝组织内的阳性表达（16．7％、16．7％和25．0％）。而且DNMTl、DNMT3a和DNMT3b与HCC的病理分化类型及乙肝表面抗原阳性显著相关（P〈0．05）。结论DNMTl、DNMT3a和DNMT3b的异常表达与HcC的发生发展有紧密的关系。

Objective To investigate the expression of DNA methyltransferase 1, 3a, 3b （DN- MT1, DNMT3a, DNMT3b） in human hepatocellular carcinoma （HCC） and to determine their clinical significance. Methods The expression of DNMT1,3a, 3b proteins was detected in 47 HCC tumor specimens and 42 HCC paracarcinoma liver tissues by immunohistoehemistry. 12 normal liver tissues were used as control. The results and clinicopathological parameters were analyzed. Results The pos- itive expression rates of DNMT1, 3a and 3b in HCC tissues were 80.9%, 68.1% and 78.7% respec- tively. The positive expression rates of DNMT1, DNMT3a and DNMT3b in paracareinoma tissues were 50.0%, 52.4% and 57. 1% respectively. The expression rates of DNMT1, 3a and 3b in both HCC tissues and paracarcinoma tissues were significantly higher than normal liver tissues. The ex- pression of DNMT1, DNMT3a, DNMT3b was correlated with tumor differentiation （P〈0.05） and hepatitis B surface antigen （HBsAg） positivity. Conclusions DNMT1, 3a and 3b play important roles in carcinogenesis and development of HCC.