透明质酸钠对骨性关节炎疗效评价的T_2 mapping方法实验研究

The Evaluation of Sodium Hyaluronate on the Treatment of Osteoarthritis with T_2 mapping

目的用T2mapping成像技术评价透明质酸钠对骨关节炎(OA)的疗效。方法取20只雌性新西兰大白兔,随机分为对照组和实验组(n=10)。在兔右膝关节股骨内髁钻深度为2 mm的软骨缺损区(r=0.5 mm)。术后第3天开始给予实验组右膝关节注射透明质酸钠0.5 ml,每周1次,共4周,对照组右膝关节及实验组左膝关节注射0.5 ml生理盐水。对照组的左膝关节不做任何处理。术后第5周处死动物进行T2mapping扫描,软骨行HE染色及Mankin评分。T2值及Mankin评分进行差异检验及相关分析(P<0.05)。结果实验组和对照组兔左膝关节的T2值分别为41.9±1.7、41.6±3.2(P>0.05),Mankin评分为0分(P>0.05)。实验组和对照组右膝关节的T2值分别为:61.1±1.1、65.2±1.2(P<0.05),Mankin评分分别为:5.6±4.9、7.1±0.5(P<0.05)。T2值和Mankin评分的相关分析显示r=0.999(P<0.05)。结论 T2mapping成像技术可以准确地评价透明质酸钠对OA的疗效。

Objective To evaluate the efficacy of HA for the treatment of OA with T2 mapping.Methods 20 New Zealand female healthy rabbits were used in this research,which were randomly divided into control group and experience group（n=10）.A circular defect（r=0.5 mm）which depth was 2 mm was made in the medial condylar of the right femoral with both control group and experience group.In the 3d day after the operation,0.5 ml HA was injected into the OA knee joint of the experience group once per week for 4 weeks.The same treatment was used to left knee of experience group instead of Sodium Chloride.All rabbits were sacrificed at 5 weeks after surgery,and T2 mapping scan was performed in all the knees,and pathological changes of articular cartilage tissue were observed with haematine eosin staining.Mankin scores was also used to evaluate the OA joint.Results The T2 value of the left knee joints in both experience group and control group was 41.9±1.7,41.6±3.2（P0.05）,respectively,the values of Mankin was 0 in two groups（P0.05）.The T2 value of the OA joint in both experience group and control group was 61.1±1.1,65.2±1.2（P0.05）,and the Mankin value was 5.6±4.9,7.1±0.5（P0.05）respectively.The correlative analysis of T2 value and Mankin was R=0.999（P0.05）.Conclusion T2 mapping can be used to evaluate the efficacy of HA for the treatment of OA.