趋化因子CCR2,CXCL9在口腔黏膜下纤维性变中的表达

Expression of chemokine CCR2,CXCL9 in oral submucous fibrosis

目的:通过检测趋化因子CCR2,CXCL9在早、中、晚期口腔黏膜下纤维性变(OSF)组织中基因及蛋白表达的改变,进一步探讨OSF的发病机制。方法:收集早、中、晚期OSF组织,RT-PCR检测趋化因子CCR2,CXCL9基因mRNA的表达,Western blot检测组织中趋化因子CCR2,CXCL9蛋白的表达。结果:趋化因子CCR2,CXCL9基因mR-NA及蛋白在早、中、晚期OSF组织中与对照组相比均呈现表达下调,在早、中、晚期病变组织中表达依次递减。在早期OSF病变组织中,CCR2,CXCL9基因mRNA较健康对照组分别上调406%和2051%,蛋白表达分别升高385%和1506%;在中期OSF病变组织中,CCR2,CXCL9基因mRNA较健康对照组分别上调369%和1901%,蛋白表达分别升高310%和1419%;在晚期OSF病变组织中,CCR2,CXCL9基因mRNA较健康对照组分别上调357%和1760%,蛋白表达分别升高309%和1401%。结论:早、中、晚OSF各期组织中趋化因子CCR2,CXCL9基因mRNA和蛋白水平表达均异常升高。此类因子的异常表达可能与OSF的发病机制相关。

Objective： To further explore the pathogenesis of oral submucous fibrosis （OSF） by testing the expressions changes of chemokine CCR2,CXCL9 in early, metaphase and advanced stage of OSF. Method：The expressions of chemokine CCR2, CXCL9 were detected by RT-PCR and Western blot techniques. Result：To compare to the control group, the mRNA of chemokine CCR2, CXCL9 increased by 406 % and 2051%,and the protein expression increased by 385 % and 1506 % in early stage of OSF;the mRNA of chemokine CCR2,CXCL9 increased by 369 % and 1901%,and the pro- tein expression increased by 310 % and 1419 % in metaphase stage：the mRNA of chemokine CCR2,CXCL9 increased by 357 % and 1760 %,and the protein expression increased by 309 % and 1401% In advanced stage. Conclusion：The gene and protein expressions of chemokine CCR2, CXCL9 were highly upregulated in different stages of OSF.The expressions changes of them maybe in relation of the pathogenesis of OSF.