人工骨颗粒大小对于牙槽骨重建影响的实验研究

Experimental Study on the Effects of Artificial Bone Particle Size for Alveolar Bone Reconstruction

目的:比较不同大小人工骨颗粒对于骨再生的引导作用。方法:将人工制备的无机骨颗粒分为直径300～500μm和850～1000μm植入GBR动物模型,分别在植入后4周及10周进行组织学观察和形态学测量。结果:术后4周时,2个实验组均可见新生骨自颅骨骨面沿人工骨颗粒向扩增区域内部生长;术后10周时,小颗粒组新生骨数量明显多于大颗粒组,且更加成熟致密,多处可见桥连样结构。新生骨面积比、新生骨与人工骨颗粒的接触长度、新生骨与人工骨颗粒之间空隙的比值、新生骨与人工骨颗粒的接触长度比,两组均随时间的延长发生显著性增加;且在术后4周与10周时,前3个指标均为小颗粒组高于大颗粒组,而接触长度比则在4周和10周时大、小颗粒组之间无显著性差异;人工骨颗粒周长和人工骨颗粒空隙比,小颗粒组在4周和10周时均显著高于大颗粒组。结论:在人工骨材料植入的早期阶段,小颗粒组较大颗粒组具有更强的骨引导再生能力。

Objective： To evaluate the effects of two different sizes of artificial bone particles on the osteoconductive function. Methods： Two different sizes including 300--5009m and 850--1000μm of artificial bone particles were used in a model of GBR. Histological examination and histomorphometric analysis were made at 4 weeks and 10 weeks after implantation. Results： Histological findings showed newly formed bone was present adjacent to the orig- inal skull surface and outgrowths to the centre of the secluded graft space in both groups at 4 weeks after implanta- tion. The created area grafted with the small particles seemed to contain more bone and interconnections than the ar- ea grafted with large particles at 10 weeks. Histomorphometrical analysis showed bone area, total bone--particle contact length, bone area ratio in interparticle space, degree of bone--particle contact increased with the time passed by. The small--particle group was significantly higher than large--particle group except the degree of bone--parti- cle contact. Both at the time of 4 weeks and 10 weeks after implantation, the small--particle group also had signifi cantly higher total particle surface length and interparticle space area than large--particle group. Conclusion： In the early stage of artificial bone particles implantation, the small particles might provide better osteoconductive function than large particles.