VEGF、MMP-9、TIMP-2在口腔颌面部鳞癌中的表达研究

Expression and significance of VEGF,MMP-9 and TIMP-2 in OSCC

目的:研究口腔鳞癌中VEGF、MMP-9和TIMP-2基因的表达与临床病理因素之间的关系及意义。方法:应用免疫组织化学Envision法检测VEGF、MMP-9和TIMP-2在50例口腔鳞癌中的表达,分析其与口腔鳞癌的临床及病理参数之间的关系。结果:VEGF在口腔鳞癌中阳性表达率与其有无淋巴结转移相关,有淋巴结转移者VEGF的表达量较无淋巴结转移者明显增加(P<0.05)。MMP-9的表达率与其临床分期及有无淋巴结转移相关;临床分期越高,MMP-9的表达越高(P<0.05);有淋巴结转移者MMP-9的表达量较无淋巴结转移者明显增加(P<0.05)。TIMP-2的阳性表达率与T分类、临床分期和有、无淋巴结转移转移有关。随着T分类、临床分期的增高,TIMP-2的表达率明显降低(P<0.05);有淋巴结转移者TIMP-2的表达较无淋巴结转移者明显降低(P<0.05)。结论:VEGF、MMP-9的高表达和TIMP-2的低表达与口腔鳞癌淋巴结转移明显相关。MMP-9的高表达和TIMP-2的低表达与口腔鳞癌的临床分期和疾病进展有一定关系。

Objective： To study the relationship between the expressions of VEGF, MMP-9 and TIMP-2 and clinic-pathologic factors as well as the clinical significance. Methods： The expression of VEGF, MMP-9 and TIMP- 2 in 50 cases of oral squamous cell carcinoma were detected by Envision immunohistochemical technique and the relationship between the expression and the clinical factors of oral squamous cell carcinoma was analyzed. Re- sults： The expression rate of VEGF in oral squamous cell carcinoma was correlated with the lymph node metastasis （P^0.05）. The expression rate was higher in the positive lymph node metastasis group than that of the negative lymph node metastasis （P〈0.05）. The expression rate of MMP--9 in oral squamous cell carcinoma was correlated with the clinical stage and lymph node metastasis （P 〈0.05）. The expression rate was higher in the higher clinical stage （P〈0. 05） and in the positive lymph node metastasis group than that of the higher clinical stage and the nega- tive lymph node metastasis group （P^0.05）. The expression rate of TIMP--2 in oral squamous cell ~arcinoma was correlated with the T--category of tumor, the clinical stage and lymph node metastasis （P^0.05）. With the higher of the clinical stage, the expression rate became lower. The expression rate was lower in the higher T-- category and the positive lymph node metastasis group than that of the lower T--category and the negative lymph node me- tastasis group （P^0.05）. Conclusion： The results indicated that the positive rate of VEGF, MMP--9 and TIMP-- 2 protein in oral squamous cell carcinoma were significantly associated with lymph node metastasis. The results in- cluced that the positive rate of MMP--9 and TIMP--2 protein were significantly correlated with tumor processes in OSCC.