高效液相色谱法测定化妆品中的维生素K1

Determination of Vitamin K1 in Cosmetics by High Performance Liquid Chromatography

建立了化妆品中维生素K1的高效液相色谱分析方法。将样品采用饱和氯化钠溶液-正己烷溶剂体系按不同的添加顺序进行分散、提取后,用高效液相色谱/二极管阵列检测器(HPLC/DAD)进行测定。其中,对油脂含量较高样品的提取液需经硅胶固相萃取柱进一步净化后测定。对不同的提取和净化方法作了系统研究,在优化实验条件下,方法的回收率为99%～105%,相对标准偏差小于5%,方法定量下限为0.4 mg/kg。该方法快速、准确、适用范围广,可用于化妆品中维生素K1含量的检测。

A high performance liquid chromatographic （HPLC） method was developed for the determination of vitamin K1 in cosmetics. Different cosmetic samples were dispersed and extracted by adding saturated sodium chloride aqueous solution and hexane in appropriate sequence, and detected by HPLC with diode array detector（HPLC/DAD）. The samples containing high level of waxes were further purified by silicagel solid phase extraction. The extraction and purification procedures were optimized. Under the optimized conditions, the average recoveries of vitamin K1 were in the range of 99% - 105% with relative standard deviations less than 5%. The limit of quantitation was 0. 4 mg/ kg. The method was accurate, rapid and widely applicable, and could be used to quantitatively analyze vitamin K1 in cosmetics.