摘要
在常用传代保藏法的基础上,进行粉拟青霉菌斜面菌种及一级固体菌种增加塑料瓶,内加2/3的蒸馏水,选用棉球塞和白橡胶塞2种试管塞并用蜡封口的菌种保藏试验。结果表明,常规与水浸2种菌种保藏31个月后,水浸白橡胶塞斜面菌种培养基无干缩现象,经室内测定,其药效达80%。
Based on traditional preserving method, new preserving method on slant strains and solid strain of Peacilomyees farinosus have been conducted in a plastic bottle within 2/3 of distilled water, using cotton balls and white rubber two kinds of tubes plug and preserved with wax seal. The results showed that after 31 months, culture medium of slant strains which using water immersion method with white rubber plug did not show shrinkage phenomenon contrasted with regular method, and its pesticide effect was up to 80% in indoor toxicity test.
出处
《林业调查规划》
2012年第5期64-66,共3页
Forest Inventory and Planning
关键词
粉拟青霉菌
菌种保藏
水浸保藏法
常规保藏法
毒力测定
Peacilomyees farinosus
culture preservation
water immersion preserving method
regular preserving method
toxicity test