肺动脉高压对内皮祖细胞与骨形成蛋白-2的影响

The effection of idiopathic pulmonary hypertension on endothelial progenitor cells and bone morphogenetic protein-2

目的分析特发性肺动脉高压(IPAH)对血浆骨形成蛋白2(BMP-2)的浓度和外周血内皮祖细胞(EPC)数量的影响以及BMP-2与肺动脉压(PAP)及EPC的关系。方法选取经右心漂浮导管检测肺动脉压确诊的IPAH住院患者28例为试验组;同时选取健康志愿者20例为对照组。采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)法测定血浆BMP-2的浓度,同时显微镜下计数外周血EPC。统计分析两组差异并对BMP-2与PAP和EPC的相关性进行分析。结果 IPAH患者血浆BMP-2水平高于对照组,差异有统计学意义[(0.1294±0.0292)μg/mlvs.(0.0898±0.0295)μg/ml,P<0.01],外周血EPC数量低于正常对照组,EPC数量具差异有统计学意义[(26.75±5.87)个vs.(42.65±8.37)个,P<0.01]。BMP-2与PAP呈正相关,与EPC数量呈负相关。结论血浆BMP-2升高及EPC数量下降可能参与IPAH的发病过程;且BMP-2与PAP和EPC均有关。

Objective To investigate the effectiong of idiopathic pulmonary arterial hypertension （ IPAH ） on bone morphogenetic protein-2 （ BMP-2 ） and counts of endothelial progenitor cells （ EPC ） , and the co relationg between and BMP-2 and pulmary arterial pressure（PAP） and the counts of EPC. Methods The patients with IPAH （ n =28 ） diagnosed by the examination of right heart floating catheters for pulmonary arterial pressure were selected as experimental group, and healthy volunteers were selected as control group. The concentration of plasma BMP-2 was detected by using enzyme-linked immunosorbent assay （ ELISA ） and EPC in peripheral blood were counted under the microscope. Results The difference in plasma BMP-2 had statistical significance between IPAN group and contrlo group [ （ 0.1294 ± 0.0292 ）μ g/mL vs.（ 0.0898 ± 0.0295 ）μ g/mL, P 0.01], and the difference in EPC counts also had statistical significance [ （ 26.75 ± 5.87 ） piece vs.（ 42.65 ± 8.37 ） piece, P 0.01]. The relation of BMP-2 and PAP was positive, while negative between BMP-2 and EPC. Conclusion IPAH caused the increase of BMP-2 while desease of EPC, the relation between BMP-2 and PAP or BMP-2 and EPC was existed in IPAH patients.