某院2010年细菌耐药性监测结果

Surveillance of bacterial resistance in the hospital of traditional Chinese medicine of Chuxiong prefecture during year 2010

目的了解2010年楚雄州中医院临床分离菌株对常用抗菌药物的耐药性。方法采用ATB药敏板条及纸片扩散法对楚雄州中医院2010年临床分离菌946株进行药敏试验。结果 946株细菌中革兰阴性菌占68.5%,革兰阳性菌占31.5%。革兰阴性菌中大肠埃希菌48.0%(331/648)、克雷伯菌属13.4%(87/648)和肠杆菌属细菌11.0%(71/648);革兰阳性菌中粪肠球菌24.5%(73/298)、金黄色葡萄球菌20.8%(62/298)和屎肠球菌18.5%(55/598)。药敏试验结果显示,铜绿假单胞菌和肠杆菌属细菌检出13.8%(8/58)和1.4%(1/71)的泛耐药菌株,未检出泛耐药的大肠埃希菌、肺炎克雷伯菌和不动杆菌;耐甲氧西林金黄色葡萄球菌和耐甲氧西林凝固酶阴性葡萄球菌分别占金黄色葡萄球菌和凝固阴性葡萄球菌的16.1%(10/62)和83.3%(40/48)。结论定期进行耐药性监测有助于了解楚雄州中医院细菌耐药性的变迁,为临床经验用药提供依据。

Objective To investigate the resistance of clinical isolates in hospital of traditional Chinese medicine of Chuxiong prefecture from January through December in 2010.Methods Antimicrobial susceptibility testing of 946 clinical isolates were carried out by Kirby-Bauer（K-B） method and microdilution method.Results Of the 946 clinical isolates,gram-negative bacteria and gram-positive bacteria accounted for 68.5% and 31.5%,respectively.Of 648 gram negative bacilli,E.coli,Klebsiella spp.and Enterobacter spp.accounted for 48.0%（331/648）,13.4%（87/648） and 11.0%（71/648）,respectively.Among 298 gram positive cocci,E.faecalis,S.aureus and E.faecium.Accounted for 24.5%（73/298）,20.8%（62/298）and 18.5%（55/298）.Susceptibility test results showed that Pseudomonas aeruginosa and Enterobacter spp.were detected in 13.8%（8/58） and 1.4%（1/71） pan-resistant strains.Not detected in the Pan-resistant E.coli,Klebsiella spp.and Acinetobacter spp.The prevalence of methicillin-resistant Staphylococcus was 83.3%（40/48） in S.aureus and 16.1%（10/62） in coagulase-negative Staphylococcus.Conclusion Surveillance of bacterial resistance is most important and valuable for guidance of rational selection of antimicrobial agents.