C-反应蛋白和淀粉样蛋白A在手足口病诊疗中的应用研究

The clinical application of CRP and SAA in the HFMD

目的探讨联合检测血清淀粉样蛋白A(SAA)和C-反应蛋白(CRP)在手足口病诊断及预后评估中的临床应用价值。方法采用荧光聚合酶链反应技术检测205例疑似手足口病患儿人肠道病毒71型核酸和柯萨奇病毒A16型核酸的感染情况;采用速率散射法测定205例疑似手足口病患儿血清CRP和SAA水平。结果手足口病组血清SAA明显高于非手足口病组,差异有统计学意义(P<0.01);CRP水平高于非手足口病组,差异有统计学意义(P<0.05);SAA与CRP比值高于非手足口病组,差异有统计学意义(P<0.05);手足口病重症组血清SAA与CRP明显高于非手足口病普通组,差异有统计学意义(P<0.01)。结论联合检测血清SAA和CRP水平及了解SAA和CRP变化情况有助于小儿手足口病的早期诊断以及危重症的早期识别。

Objective To explore the clinical application of C-reactive protein（CRP） and serum amyloid A（SAA） in hand-foot-and-mouth disease（HFMD）.Methods 205 patients with suspected HFMD symptoms were enrolled into this study.EV71-RNA and CA16-RNA were measured by fluorescence RT-PCR.Serum CRP and SAA were parallelly measured with nephelometry.Results In term of SAA,statistic analysis showed that there was significant difference between HFMD group and non-HFMD group（P0.01）.As for CRP,there was statistic difference between HFMD group and non-HFMD group（P0.05）.As far as SAA/CRP ratio,there was not only statistic difference between HFMD common group and HFMD crises group（P0.05）,but also significant difference between HFMD crises group and non-HFMD group（P0.01）.Conclusion The reports showed that test SAA combined with CRP,and knew the variation of SAA/CRP,would did great help to diagnose of HFMD and detect the crises of HFMD early.