摘要
利用MDBK传代细胞系对湖北通山县某羊场疑似羊口疮(Orf)的黑山羊病料进行分离,通过病毒理化特性试验、动物回归试验和PCR等方法对分离的毒株进行鉴定。结果表明,病料悬液接种MDBK细胞后出现细胞病变,接种病料的羊只出现典型的Orf症状,扩增出羊口疮病毒(Orf virus)B2L基因,该毒株与台湾山羊株(EU935106.1、DQ904351.1)相似性最高(均为99%),亲缘关系最近。本研究成功分离鉴定到一株ORFV,命名为ORFV/HB/09。
The virus strain was isolated from crust materials of black lambs with Orf like-clinical symptoms from a goat farm in Tongshan county of Hubei province by passage in MDBK cells.The virus was identified as Orf virus using the animal regression test and PCR method.The results showed that MDBK cell cultures infected with crust materials displayed cytopathic effects.Animals inoculated with crust materials occurred typical symptoms of Orf symptoms.The specific Orf virus B2L gene was amplified.The phylogenetic tree analysis demonstrated that the isolated strain was closest to similarity of nucleotide sequences（99%） with those of Taiwan strains（EU935106.1,DQ904351.1）.It was concluded that the Orf virus strain was successfully isolated and identified,named ORFV/HB/09.
出处
《动物医学进展》
CSCD
北大核心
2012年第11期37-40,共4页
Progress In Veterinary Medicine
基金
国家现代肉羊产业技术体系项目(CARS-39)
