禽腺病毒载体研究进展

Progress on Fowl Adenovirus Vector

人腺病毒载体被广泛地用作转基因工具。然而,人体内预先存在的针对人腺病毒的抗体影响了转基因的效率,阻碍了这些载体在临床上的应用。避免宿主免疫反应的途径之一是开发能将基因转导至人细胞的动物腺病毒载体,而禽腺病毒载体是目前动物腺病毒载体研究中较多的一种。禽腺病毒载体还可插入并表达病原的保护性抗原基因,用于疫苗研制。对禽腺病毒的分子生物学和载体疫苗的深入研究,将对禽类疫病预防起到重要作用。论文详细叙述了禽腺病毒的基因组结构特点以及禽腺病毒载体的研究现状,为研究者提供参考。

Human adenovirus vectors are extensively used as gene transfer vehicles.However,a serious obstacle for the use of these vectors in clinical applications is due to preexistent immunity to human adenovirus affecting the efficacy of gene transfer.One of the approachs to circumvent host immune response could be the development of vectors based on animal adenovirus that are able to transduce genes into human cells,and research work on fowl adenovirus vectors was extensively made currently.Fowl adenovirus vectors could also be used for vaccine development as they were capable of expressing an inserted gene of pathogenic protective antigen.Indepth research on molecular biology and vaccine development of fowl adenovirus would play an important role in prevention of avain diseases.The paper reviewed research progress on the construction of fowl adenovirus vectors in detail as well as genome structure and characterization of fowl adenovirus,which provides references for researchers.