男性不育患者精液活性氧和精浆超氧化物岐化酶活性测定的临床价值研究

Clinical significance of semen reactive oxygen species and seminal plasma superoxide dismutase activity detection in infertile patients

目的探讨精液ROS、精浆SOD测定在男性不育患者中临床价值。方法选择有生育力的男性健康对照组50例,男性不育症组265例,化学发光法测定精液ROS,黄嘌呤氧化酶法测定精浆SOD活性。结果男性不育组、精索静脉曲张组和白细胞精子症组:精液ROS均明显高于对照组精液ROS,精浆SOD均明显低于对照组精浆SOD,以上组间结果差异都具有统计学意义(P<0.01)。各弱精子症组:精液ROS均明显高于对照组(P<0.01),精浆SOD均明显低于对照组(P<0.01)。各弱精子症组随着精子活力降低:精液ROS依次升高(组间结果差异有显著性意义,P<0.01);精浆SOD依次降低,其中中度弱精子症组精浆SOD与轻度弱精子症组相比较,结果无统计学意义(P>0.05),重度弱精子症组精浆SOD明显低于中度弱精子症组和轻度弱精子症组,组间结果差异有统计学意义(P<0.01)。结论ROS是影响男性不育的一个重要病因和病理因素,男性不育患者精液ROS和精浆SOD检测具有重要的临床意义。

Objective To investigate the clinical significance of semen ROS and seminal plasma SOD activity in male infertility. Methods Semen ROS and seminal plasma SOD of 50 cases from fertile male as the healthy control group and 265 patients with male infertility as the test group were detected by chemiluminescence method and xanthine oxidase method respectively.Results The levels of semen ROS and seminal plasma SOD in male infertility group,varieocele group and leukoeytospermia group were significantly different from that of the healthy control group （P〈0.01）.The levels of semen ROS in each asthenospermia group were significantly higher than that of the healthy control group （P〈0.01）and the levels of seminal plasma SOD in each asthenospermia group were significantly lower than the control group（P〈0.01）.The levels of semen ROS in each asthenospermia group increased （P〈0.01）and the level of seminal plasma SOD decrease with the decreased sperm motility.There was no statistical significance（P〉0.05） between the levels of seminal plasma SOD in moderate asthenospermia group and slight asthenospermia group.The levels of seminal plasma SOD in severe asthenospermia group was significantly lower than that of moderate asthenospermia group and mild asthenospermia group （P〈0.01）. Conclusion ROS is an important etiological and pathological factor in male infertility. The levels of sperm ROS and seminal plasma SOD have important clinical significance.