南方汉族急性髓细胞白血病患者杀伤细胞免疫球蛋白样受体基因的研究

Study on the correlation of killer immunoglobin-like receptor gene diversity with acute myeloid leukemia in the southern Chinese Han population

目的研究和分析南方汉族急性髓细胞白血病(acute myeloid leukemia,AML)患者杀伤细胞免疫球蛋白样受体(KIR)的基因频率和单倍型。方法采用序列特异性引物PCR(polymerase chain reaction with sequence-specific primers,PCR-SSP)技术对南方地区76例急性髓细胞白血病患者进行KIR基因的检测和分析,并与77例随机健康对照人群KIR基因频率进行比较。结果共检出16个KIR基因;在AML患者中检出15种基因型,正常人群中检出18种基因型。结论与正常人比较,AML患者各KIR基因频率无显著性差异。

Objective To investigate the distribution of KIR gene and haplotype in acute myeloid leukemia of southern Chinese Han population. Methods A total of 76 patients with acute myeloid leukemia （AML） and 77 randomly-selected healthy controls in the southern Chinese Han individuals were subjected to KIR PCR-SSP genotyping using the inno-train commercial kit. Results The frequency of KIR was not significantly different when compared to the controls. We identified a total of 15 different KIR genotypes in AML patients and 18 in the control individuals. Conclusion There is no significiant difference between KIR and AML in the southern Chinese Han individuals.