葡萄糖异构酶的双层固定化及其在高果糖浆工业化生产中的应用

Bilayer-Immobilization of Glucose Isomerase and Its Application in Production of High Fructose Syrup

在对多孔三甲铵甲基聚苯乙烯 (TMPS)载体的合成研究基础上 ,运用分子沉积技术 ,完成葡萄糖异构酶 (GI) 2 0 0 kg双层固定化试验 ,并在万吨生产线上进行 1 70 0 t果葡糖浆生产试验 .结果表明 ,开发的双层固定化葡萄糖异构酶 (IGI)活力可达到 2 2 0 0 μmol/ min·g干胶 ,活力回收率达 68.5 % .载体机械强度良好 ,双层 IGI的活力比单层 IGI活力提高 1 1 4% ,IGI的半衰期 45 d左右 ,3个半衰期的转化力为 1 1 840 kg干基葡萄糖 / kg IGI.这一结果与普通 IGI相比 ,相同高果糖浆生产能力的异构化装置可缩小 1倍 ,载体用量可减少 1倍 .

A molecular deposition technique was applied to glucose isomerase immobilization that resulted in its doubled activity. Bilayer glucose isomerase was immobilized in porous \%p\%\|trimethylamine\|polystyrene(TMPS) beads. We completed 200 kg bilayer IGI with the activity of 2 200 μmol/min\5g, and it was applied to production line test(1 700 t). Through chemical analysis, we found the activity of bilayer IGI was 114% higher than that of normal IGI. During immobilization, the recovery activity of initially added glucose isomerase was 68.5%. The half\|life of the bilayer IGI was approx 45 d at pH 8.5 and 60 ℃, with 40% glucose as substrate. The specific productivity of bilayer IGI was 1 840 kg dry glucose/kg dry IGI. This result means that the construction cost of a new HFCS plant and its running fee for glucose to fructose could be half declined.