红掌组培快繁技术研究

Study on Technology of Tissue Culture and Rapid Propagation of Anthurium andraeanum

以红掌的顶芽、茎段、叶片、叶柄等为外植体,通过以芽繁芽方式或愈伤组织诱导芽途径诱导无菌芽,并获得再生植株,以期建立一套完善的红掌组培快繁技术体系。结果表明:改良MS+6-BA 2.0mg/L+NAA 0.5mg/L培养基组合最适合红掌以芽繁芽的增殖芽,繁殖系数达4.5以上;而改良MS+6-BA 2.0mg/L+KT 1.0mg/L培养基组合更适合愈伤组织分化芽和增殖芽,芽繁殖系数达5.8以上;最好的生根培养基为1/2MS+NAA 0.5mg/L。

With teminal bud,stem,leaf stall,leaf as explants,the tissue culture and rapid propagation of Anthurium andraeanum were stiudied,by the way of bud proliferation or bud induction from the callus.The results showed that the optimal medium for bud proliferation was modifiede MS＋6-BA 2.0 mg/L＋NAA 0.5 mg/L,whose proliferation coefficient reached 4.5;the optimal medium for bud induction from the callus was modified MS＋6-BA 2.0 mg/L＋KT 1.0 mg/L,and the proliferation coefficient more than 5.8;the optimal medium for rooting was 1/2 MS＋NAA 0.5 mg/L.