摘要
对甘南藏族自治州尖顶羊肚菌进行液体发酵培养,以干菌丝体得率为指标,设计正交试验优化其液体发酵培养基和培养条件。实验结果表明最佳培养基组合为白砂糖30 g.L-1、豆粕粉15 g.L-1、KH2PO40.75 g.L-1、MgSO4.7H2O 0.5g.L-1、维生素B10.03 g.L-1。最优液体发酵条件为温度25℃,培养液初始pH7,接种量10 mL,转速120 r.min-1,培养时间8 d。
The medium and liquid fermentation conditions of Morchella conica which growed in Gannan Tibetan au- tonomous prefecture was studied in the paper as the index to dry mycelia yield by orthogonal experiment. The results showed that the best medium components was sugar （30g·L^-1）, soybean powder （15 g·L^-1）, KH2PO4 （0.75 g·L^-1）, Mg- SO4·7H2O （0.5 g·L^-1） and vitamin Bl （0.03 g·L^-1）. The optimal conditions of liquid fermentation were showed as follows： the temperature was 25℃, the initial pH was 7, the strains for inoculation quantity was 10 mL, the rotate speed was 120 r· min^-1 and culture time was 8 days.
出处
《中国食用菌》
2012年第6期40-43,共4页
Edible Fungi of China
关键词
甘南藏族自治州
尖顶羊肚菌
液体发酵
Gannan tibetan autonomous prefecture
Morchella conica
Liquid fermentation
