摘要
量子点(QD)具有抗漂白能力强、量子产率高、激发谱宽和发射谱窄等优点,在生物荧光标记领域有较广泛的应用。利用QD 585和Hoechst 33342(H342)双标记小鼠卵母细胞中DNA甲基转移酶(Dnmt)和染色体,通过双光子成像同时双通道探测它们的荧光图像,获得了Dnmt蛋白表达的三维空间分布。发现老龄小鼠卵母细胞不适合体外培养成熟:该成熟方式不仅引起老龄小鼠卵母细胞Dnmt蛋白含量的变化,而且还改变了它们在胞质中的空间分布。这些改变可能与异常的甲基化修饰有关。
Quantum dot （QD） is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases （Dnmts） and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors＇ results showed that aging mouse oocytes may be not suitable for in-vitro maturation： both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA meth- ylation modification.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2012年第12期3175-3178,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(90919012)
国家教育部博士点专项基金项目(20090002110065)
清华大学自主科研计划项目(2010THZ01)资助
